International Journal on Magnetic Particle Imaging IJMPI
Vol. 12 No. 1 Suppl 1 (2026): Int J Mag Part Imag
https://doi.org/10.18416/IJMPI.2026.2603009

Proceedings Articles, ID 996

MPI quantification of magnetically labeled extracellular vesicles in mouse heart following myocardial infarction

Main Article Content

Wenshen Wang (1) F.M. Kirby Research Center, Kennedy Krieger Institute, 2) Department of Radiology, Johns Hopkins University;), Zheng Han (Department of Biomedical Engineering, University of Central Oklahoma, Edmond, Oklahoma, USA), Safiya Aafreen (Department of Biomedical Engineering, Johns Hopkins University), Cristina Zivko (Department of Genetic Medicine, Johns Hopkins University), Olesia Gololobova  (Department of Molecular and Comparative Pathobiology, Johns Hopkins University), Vasiliki Machairaki (Department of Genetic Medicine, Johns Hopkins University), Kenneth Witwer (Department of Molecular and Comparative Pathobiology, Johns Hopkins University), Geoffrey Cotin (SuperBranche), Delphine Felder-Flesch (SuperBranche), Jeff Bulte (1) Department of Radiology, 2) Department of Biomedical Engineering, 3) Cellular Imaging Section and Vascular Biology Program, Institute for Cell Engineering, Johns Hopkins University; 4) F.M. Kirby Research Center, Kennedy Krieger Institute;), Robert Weiss (Department of Medicine, Johns Hopkins University), Guanshu Liu (1) F.M. Kirby Research Center, Kennedy Krieger Institute; 2) Department of Radiology, Johns Hopkins University)

Copyright (c) 2026 Wenshen Wang, Zheng Han, Safiya Aafreen, Cristina Zivko, Olesia Gololobova , Vasiliki Machairaki, Kenneth Witwer, Geoffrey Cotin, Delphine Felder-Flesch, Jeff Bulte, Robert Weiss, Guanshu Liu

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

This study aims to develop magnetically labeled iPSC-derived extracellular vesicles (magneto-EVs) for MRI/MPI and myocardial repair in a theranostic manner. Using optimized electroporation and purification protocols, we prepared magneto-EVs with high SuperSPIO20 loading efficiency (1.77 ng Fe/10? EVs) and well-preserved therapeutic effects. In vitro MPI results reveal that the detectability of magneto-EVs is ~1 × 109 EVs/mL. In vivo MPI shows that MPI can detect magneto-iPSC-EVs injected intramyocardially in mouse hearts for up to 7 days following myocardial infarction. MPI quantification showed that 32% of injected EVs remained in the heart after 24 hours, with a further ~50% decrease by day 7, indicating progressive clearance or biodegradation of magneto-EVs from the injected site. Despite this low retention and rapid clearance, magneto-EVs led to significant improvement in left ventricular ejection fraction (37.3% increase) and scar formation (61.0% reduction). This theranostic EV imaging platform enables quantitative MPI monitoring of EV delivery and predicting therapeutic outcomes.

Article Details

References

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