International Journal on Magnetic Particle Imaging IJMPI
Vol. 12 No. 1 Suppl 1 (2026): Int J Mag Part Imag
https://doi.org/10.18416/IJMPI.2026.2604007

Research Articles, ID 1040

Establishing stable antibody-magnetic nanoparticle conjugates: A methodological study

Main Article Content

Anja Stähle (Department of Rheumatology and Clinical Immunology, University of Lübeck, Lübeck, Germany), Maria-Josephina Buhné (Institute of Radiology and Nuclear Medicine, University Hospital Schleswig-Holstein, Lübeck, Germany), Jessica Schnohr (Department of Rheumatology and Clinical Immunology, University of Lübeck, Lübeck, Germany), Joana Besecke (Institute of Interventional Radiology, University Hospital Schleswig-Holstein, Lübeck, Germany), Peter Lamprecht (Department of Rheumatology and Clinical Immunology, University of Lübeck, Lübeck, Germany), Susanne Schinke (Department of Rheumatology and Clinical Immunology, University of Lübeck, Lübeck, Germany), Gabriela Riemekasten (Department of Rheumatology and Clinical Immunology, University of Lübeck, Lübeck, Germany), Helge Mueller-Fielitz (Institute of Experimental and Clinical Pharmacology and Toxicology, University of Lübeck, Lübeck, Germany), Jan Wenzel (Institute of Experimental and Clinical Pharmacology and Toxicology, University of Lübeck, Lübeck, Germany), Roman Klöckner (Institute of Interventional Radiology, University Hospital Schleswig-Holstein, Lübeck, Germany), Jörg Barkhausen (Institute of Radiology and Nuclear Medicine, University Hospital Schleswig-Holstein, Lübeck, Germany), Thorsten M. Buzug (1) Institute of Medical Engineering, University of Lübeck, Lübeck, Germany 2) Fraunhofer IMTE, Fraunhofer Research Institution for Individualized Medical Technology and Engineering, Lübeck, Germany), Hanna Graßhoff (Department of Rheumatology and Clinical Immunology, University of Lübeck, Lübeck, Germany), Franz Wegner (1) Institute of Interventional Radiology, University Hospital Schleswig-Holstein, Lübeck, Germany 2) Fraunhofer IMTE, Fraunhofer Research Institution for Individualized Medical Technology and Engineering, Lübeck, Germany), Mandy Ahlborg (Fraunhofer IMTE, Fraunhofer Research Institution for Individualized Medical Technology and Engineering, Lübeck, Germany)

Copyright (c) 2026 Anja Stähle, Maria-Josephina Buhné, Jessica Schnohr, Joana Besecke, Peter Lamprecht, Susanne Schinke, Gabriela Riemekasten, Helge Mueller-Fielitz, Jan Wenzel, Roman Klöckner, Jörg Barkhausen, Thorsten M. Buzug, Hanna Graßhoff, Franz Wegner, Mandy Ahlborg

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Antibody-conjugated magnetic nanoparticles offer considerable potential for morphological and functional assessment of vascular pathologies using Magnetic Resonance Imaging or Magnetic Particle Imaging. However, reliable antibody-nanoparticle conjugation remains technically challenging and strongly depends on the applied coupling chemistry. In this work, different maleimide-based conjugation strategies were evaluated to establish stable antibody attachment to amine-functionalized magnetic nanoparticles. This study highlights the critical impact of linker chemistry on antibody-nanoparticle conjugation and provides a methodological basis for further functional and imaging-based investigations with magnetic particle imaging.

Article Details

References

[1] H. Graßhoff et al. Autoantibodies as Biomarker and Therapeutic Target in Systemic Sclerosis. Biomedicines, 10(9), ID 2150, 2022, DOI:
10.3390/biomedicines10092150
[2] E. Aderhold et al., Development and Assessment of a 1D-MPS, IJMPI,11(1), ID 871, 2025.

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