International Journal on Magnetic Particle Imaging IJMPI
Vol. 8 No. 1 Suppl 1 (2022): Int J Mag Part Imag
https://doi.org/10.18416/IJMPI.2022.2203034
A magnetic immunization method based on magnetic nanomarkers under the DC bias field
Main Article Content
Copyright (c) 2022 Xinchao Cui
This work is licensed under a Creative Commons Attribution 4.0 International License.
Abstract
This paper investigates the effect of the SNR of the magnetic response harmonic signal on the sensitivity of biomolecular detection. A method is proposed for measuring the 2nd and 3rd harmonics of magnetic nanotags under a DC bias field, avoiding the influence of antimagnetic and paramagnetic substances around the magnetic nanotags, as well as the background magnetic field, on the 1st harmonic signal. Specific streptavidin-modified magnetic nanoparticles were used as measurement objects, and their binding to biotinylated polystyrene microspheres was measured by a prepared detection device to simulate the detection of specific biomolecules. Using the 2nd to 3rd harmonic ratio, the lower limit of detection of the lowest 4amol (0.4pM) polystyrene microspheres in a 100uL solution was eventually determined.