International Journal on Magnetic Particle Imaging IJMPI
Vol. 12 No. 1 Suppl 1 (2026): Int J Mag Part Imag
https://doi.org/10.18416/IJMPI.2026.2603014

Proceedings Articles, ID 1023

Detection of Pseudomonas aeruginosa in Whole Blood using a portable Magnetic Particle Spectrometer - a feasibility study

Main Article Content

Lukas Heinen (Department of Otorhinolaryngology – Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner Fresenius Foundation Professorship (EKFS), Universitätsklinikum Erlangen, Erlangen, Germany), Alexander Simonis (1) Department I of Internal Medicine, Faculty of Medicine and University Hospital Cologne, Cologne, Germany; 2)Center for Molecular Medicine Cologne, Faculty of Medicine and University Hospital Cologne, University of Cologne, Cologne, Germany; 3) German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, Germany), Jan Rybniker (1) Department I of Internal Medicine, Faculty of Medicine and University Hospital Cologne, Cologne, Germany; 2)German Center for Infection Research (DZIF), Partner Site Bonn-Cologne, Cologne, Germany), Johanna Günther (Department of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Würzburg, Germany), Patrick Vogel (Department of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Würzburg, Germany), Christina Janko (Department of Otorhinolaryngology – Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner Fresenius Foundation Professorship (EKFS), Universitätsklinikum Erlangen, Erlangen, Germany), Christoph Alexiou (Department of Otorhinolaryngology – Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner Fresenius Foundation Professorship (EKFS), Universitätsklinikum Erlangen, Erlangen, Germany), Stefan Lyer (Department of Otorhinolaryngology – Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner Fresenius Foundation Professorship (EKFS), Universitätsklinikum Erlangen, Erlangen, Germany), Rainer Tietze (Department of Otorhinolaryngology – Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner Fresenius Foundation Professorship (EKFS), Universitätsklinikum Erlangen, Erlangen, Germany)

Copyright (c) 2026 Lukas Heinen, Alexander Simonis, Jan Rybniker, Johanna Günther, Patrick Vogel, Christina Janko, Christoph Alexiou, Stefan Lyer, Rainer Tietze

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Rapid identification of WHO critical priority pathogens, such as Pseudomonas aeruginosa, is essential for managing sepsis, where standard blood cultures cause diagnostic delays of 24-72 hours. To address this, we developed a rapid, culture-independent magnetic immunoassay using a portable Magnetic Particle Spectroscopy (MPS) device for the direct detection of P. aeruginosa in blood. We evaluated two SPION formulations and found that our developed formulation, SPION-152, demonstrated excellent specificity, generating a strong signal change with virulent P. aeruginosa strains while showing negligible cross-reactivity with E. coli. Specificity for a virulence-associated factor was confirmed using a knockout mutant. These findings validate the MPS platform as a promising tool for accelerating pathogen identification in patients with suspected sepsis.

Article Details

References

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