International Journal on Magnetic Particle Imaging IJMPI
Vol. 9 No. 1 Suppl 1 (2023): Int J Mag Part Imag
https://doi.org/10.18416/IJMPI.2023.2303063

Proceedings Articles

Fast detection of Sars-CoV2 Antibodies by the use of critical off-set magnetic particle spectroscopy (COMPASS)

Main Article Content

Bernhard Friedrich (Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Department of Example Science, Germany), Rainer Tietze (Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Department of Example Science, Germany), Martin Rückert (Department of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Germany), Thomas Kampf (Department of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Germany), Thomas Hennig (Institute for Virology and Immunobiology, Julius-Maximilians-University Würzburg, Germany), Lars Dölken (Institute for Virology and Immunobiology, Julius-Maximilians-University Würzburg, Germany), Volker Behr (Department of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Germany), Christoph Alexiou (Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, University Hospital Erlangen, Department of Example Science, Germany), Patrick Vogel (Department of Experimental Physics 5 (Biophysics), Julius-Maximilians-University Würzburg, Germany), Stefan Lyer (Department of Otorhinolaryngology, Head and Neck Surgery, Section of Experimental Oncology and Nanomedicine (SEON), Professorship for AI-controlled Nanomaterials, University Hospital Erlangen, Germany)

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Abstract

Current bioassays for detection of antibodies or antigens such as ELISA (Enzyme-linked Immunosorbent Assay) are relatively inflexible, expensive and time-consuming. Upcoming methods, such as ACS (AC susceptometry) or MPS (Magnetic Particle Spectroscopy), exploit the magnetization response of functionalized MNP ensembles to assess specific information about the MNP mobility as well as conjugations of chemical or biological compounds on their surface. Both methods have shown promising results in the past but cannot reach the sensitivity of above-mentioned techniques. We used a novel method based on a modified MPS being sensitive to minimal changes in mobility of MNP ensembles. This facilitates robust and easy-to-handle measurements of minimal changes in the diameter of MNPs. As an example, we detected SARS-CoV-2 antibodies binding to the S1 antigen on the surface of functionalized MNPs. Without any purification or incubation, we could show a sensitivity of less than 50 ng/mL of SARS-CoV-2 antibodies in human blood.

Article Details

References

[1] Ali, A. et al. Review on recent progress in magnetic nanoparticles: synthesis, characterization, and diverse applications. Front. Chem.9, 629054 (2021).
[2] K. Wu et al., Magnetic nanoparticles and magnetic particle spectroscopy-based bioassays: a 15-year Recap, Nano Futures, vol. 6(2):022001, Apr. 2022.
[3] P. Vogel et al., Critical Offset Magnetic PArticle SpectroScopy for rapid and highly sensitive medical point-of-care diagnostics, Nature Communications, vol. 13(1):7230, Nov. 2022.
[4] Friedrich, B. et al. Scavenging of bacteria or bacterial products by magnetic particles functionalized with a broad-spectrum pathogen recognition receptor motif offers diagnostic and therapeutic applications. Acta Biomater 141, 418-28 (2022).

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