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Copyright (c) 2022 K. L. Barry Fung, Prashant Chandrasekharan, Xinyi Y. Zhou, Weiwen Cui, Lawrence Fong, Steven Conolly
This work is licensed under a Creative Commons Attribution 4.0 International License.
White blood cell (WBC) tracking is an imaging technique for clinical diagnoses of inflammation. WBC tracking utilises In-111 scintigraphy with ex vivo WBC labeling, which is cumbersome (~1 hr prep), requires hot chemistry, and kills most WBCs within 24 hours. Magnetic particle imaging (MPI) is a tracer imaging modality that detects superparamagnetic iron oxide nanoparticles (SPIOs), providing a sensitive (~200 cell), radiation-free alternative. As such, this work shows the first in situ labelling and tracking of WBCs to lipopolysaccharide-induced myositis using antibody-targeted MPI (Ab-MPI). SPIOs functionalized with Anti-Ly6G or Anti-F4/80 antibodies were used to track neutrophils or macrophages respectively, yielding differential dynamics and contrast (CNR = ~8-13) in imaging myositis vs. an untargeted control (ferucarbotran). These results showcase Ab-MPI as a radiation-free imaging platform for in situ targeting of immune cell speicific antigen epitopes.
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